Compositions and methods to improve skin quality and appearance, cure skin and tissue damage, and use in therapy

A method of treatment of skin of a mammal in need thereof, wherein the skin is affected with a skin condition, comprising:

• generating a combined conditioned media preparation comprising a first set of growth factors and a second set of growth factors by initiating a cell culture by contacting a cell culture media with a cell population consisting of a plurality of pluripotent stem cells;
• initiating differentiation of the plurality pluripotent stem cells by adding a differentiation media to the cell culture to induce differentiation of pluripotent stem cells of the cell culture, thereby generating a differentiating stem cell culture;
• incubating the differentiating stem cell culture until a first time point, wherein differentiation of the plurality of pluripotent stem cells generates a first distinct cell population consisting of cells a first distinct cell type that are in a first distinct differentiation state at the first time point;
• collecting a first conditioned media comprising the first set of growth factors from the differentiating stem cell culture at the first time point;
• storing the first conditioned media;
• adding a volume of the differentiating media to the differentiating stem cell culture;
• collecting a second conditioned media from the differentiating stem cell culture at a second time point following addition of the differentiating media, wherein cells of differentiating stem cell culture consisting of cells of a second distinct cell type that are in a second differentiation state at the second time point;
• combining the first conditioned media and the second conditioned media to generate the combined conditioned media;
• and
• topically administering a composition comprising the combined conditioned media and a pharmaceutically or cosmetically acceptable carrier suitable for topical administration in an amount and under a protocol effective to treat the condition.