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US 11,740,236 B2Granted
Protein for rapid, efficient capture of antigens
AssigneeMassachusetts Institute of Technology
InventorsHadley Sikes Johnson, Eric Alexander Miller, Ki-Joo Sung
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Abstract
The present disclosure relates to proteins comprising a target-binding domain for detection of a target of interest, methods, compositions and kits thereof.
Claims7
A method for detecting an antigen of interest, the method comprising:
- (a) contacting a bifunctional fusion protein comprising a cellulose binding domain (CBD) and an engineered reduced-charge Sso7d (rcSso7d) antigen-binding protein with a cellulose-containing substrate for a time sufficient for the bifunctional fusion protein to bind the cellulose-containing substrate, wherein the rcSso7d antigen-binding protein comprises an amino acid sequence that is at least 85% identical to SEQ ID NO:
- 3 and has a reduced charge relative to SEQ ID NO:
- 12, and wherein the CBD comprises an amino acid sequence that is at least 85% identical to SEQ ID NO:
- 2, SEQ ID NO:
- 16, or SEQ ID NO:
- 10;
- (b) contacting the bifunctional fusion protein bound to the cellulose-containing substrate with a sample comprising an antigen of interest;
- and
- (c) detecting the antigen of interest bound by the engineered rcSso7d antigen-binding protein.
6 more elements
Dependencies are inferred from the claim text.