Biomarkers for detection of burkholderia pseudomallei
AssigneeSRI International
InventorsThomas A. Shaler, Hua Lin
Life of this patent
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Abstract
Various examples are directed to kits, apparatuses, and methods for determining a presence of Burkholderia pseudomallei (BP) in a biological sample. An example method includes causing a physical interaction between a biological sample from a subject and a set of first agents by exposing the biological sample to the set of first agents, the set of first agents being specific to one or more of a set of BP biomarkers associated with one or more proteins released from BP or associated with other molecules released from BP. The method further includes determining a presence of BP in the biological sample based on detected binding between one or more of the set of first agents and the one or more of the set of BP biomarkers.
Claims20
A method comprising:
- causing a physical interaction between a biological sample from a subject and a set of first agents by exposing the biological sample to the set of first agents, the set of first agents being specific to a set of Burkholderia pseudomallei (BP) biomarkers associated with proteins released from BP or associated with other molecules released from BP, wherein the set of BP biomarkers is associated with at least a chitin-binding protein BPSS0493 and a protein BPSL1622;
- and
- determining a presence of BP in the biological sample based on detected binding between the one or more of the set of first agents and the one or more of the set of BP biomarkers within twenty-four hours of causing the physical interaction.
A kit for detecting a presence of Burkholderia pseudomallei (BP), the kit comprising:
- a solid support;
- a set of first agents to bind to a set of BP biomarkers associated with proteins released from BP or associated with other molecules released from BP as present in a biological sample obtained from a subject, wherein the set of BP biomarkers is associated with at least a chitin-binding protein BPSS0493 and a protein BPSL1622;
- and
- a set of labels to bind to the proteins, the solid support to receive application of the biological sample, the set of first agents, and the set of labels, and in response to the application, a presence of the BP biomarker causes accumulation of one or more of the set of labels bound to the solid support and indicates the presence of BP in the biological sample within twenty-four hours of the application of the biological sample.
An apparatus comprising:
- reagents including a set of first agents and a set of second agents;
- and
- a solid support including:
- a first region to receive a biological sample obtained from a subject;
- a second region including the set of first agents, each bound to a label, the set of first agents being adherent on the second region and being specific to a set of Burkholderia pseudomallei (BP) biomarkers associated with proteins released from BP or associated with other molecules released from BP, wherein the set of BP biomarkers is associated with at least a chitin-binding protein BPSS0493 and a protein BPSL1622;
- and
- a third region including the set of second agents immobilized to the third region and being specific to the proteins,
- wherein in response to receipt of the biological sample,
- the first region is to pass a portion of the biological sample to the second region, the second region is to pass at least some of the portion of the biological sample to the third region;
- and
- the presence of the BP biomarker causes accumulation of the label in the third region indicating a presence of BP in the biological sample within twenty-four hours from application of the biological sample.
Dependencies are inferred from the claim text.