Enzymatic method for preparation of UDP-galactose
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsReza Mahour, Thomas F. T. Rexer
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Abstract
The present invention relates to an enzyme-catalyzed process for producing UDP-galactose from low-cost substrates uridine monophosphate and D-galactose in a single reaction mixture. The process can be operated (semi)continuously or in batch mode. The process can be extended to uridine as starting material instead of uridine monophosphate. Further, the process can be adapted to produce galactosylated molecules and biomolecules including saccharides, proteins, peptides, glycoproteins or glycopeptides, particularly human milk oligosaccharides (HMO) and (monoclonal) antibodies.
Claims15
A method for producing uridine 5 ′-diphospho-a-D-galactose comprising the following steps:
- A) providing a solution comprising
- (i) uridine monophosphate and D-galactose represented by the following formulae
- (ii) polyphosphate, and adenosine triphosphate;
- and
- providing a set of enzymes comprising a glucose-1-phosphate uridylyltransferase, a galactokinase, a polyphosphate kinase, and a uridine monophosphate kinase;
- B) producing uridine 5 ′-diphospho-α-D-galactose from uridine monophosphate and D-galactose in the presence of the set of enzymes, polyphosphate, and adenosine triphosphate.
Dependencies are inferred from the claim text.