Recombinant microorganism for producing citicoline and method for producing citicoline

A genetically modified Escherichia coli for producing citicoline comprising the following modifications:

• a. enzymes involved in reuse of citicoline, or choline, or phosphocholine being disrupted, wherein the enzymes comprise cytidine-5′-diphosphoinositol hydrolase, cytidine-5′-diphosphate-diacylglycerol pyrophosphatase, choline dehydrogenase, alkaline phosphatase or acid phosphatase;
• b. choline kinase for catalyzing choline chloride to generate phosphocholine being overexpressed, wherein the choline kinase comprises CKI1 or EKI1 obtained from Saccharomyces cerevisiae, or LicC obtained from Streptococcus;
• c. cytidylyltransferase for catalyzing phosphocholine to generate citicoline being overexpressed, wherein the cytidylyltransferase comprises PCT1, CDS1, or ECT1 obtained from Saccharomyces cerevisiae;
• or CdsA, IspD, MocA, KdsB, or Cca obtained from Escherichia coli;
• or LicC obtained from Streptococcus;
• or CD36_40620 obtained from Candida dubliniensis;
• d. choline transporter protein, for transporting choline chloride into a cell being overexpressed, wherein the choline transporter protein comprises BetT obtained from Escherichia coli;
• and
• e. uridine-5′-monophosphate phosphorylase for degrading uridine-5′-monophosphate into uridine is disrupted;
• and the uridine-5′-monophosphate phosphorylase comprises UmpH, UmpG, PhoA, AphA, or YjjG.