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US 11,739,355 B2Granted
Engineered biosynthetic pathways for production of histamine by fermentation
AssigneeZymergen Inc.
InventorsCara Ann Tracewell, Alexander Glennon Shearer, Michael Shareef Siddiqui, Steven M. Edgar, Nicolaus Herman, Murtaza Shabbir Hussain
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Abstract
The present disclosure describes the engineering of microbial cells for fermentative production of histamine and provides novel engineered microbial cells and cultures, as well as related histamine production methods.
Claims15
An engineered fungal cell that expresses:
- a non-native histidine decarboxylase having at least 70% amino acid sequence identity with a histidine decarboxylase from Chromobacterium sp. LK1 having SEQ ID NO:6 or from Acinetobacter baumannii strain AB0057 having SEQ ID NO:1;
- and
- at least one ATP phosphoribosyltransferase, wherein the ATP phosphoribosyltransferase comprises:
- an ATP phosphoribosyltransferase having at least 70% amino acid sequence identity with an ATP phosphoribosyltransferase from S. cerevisiae S288c having SEQ ID NO:3;
- and/or
- a feedback-deregulated ATP phosphoribosyltransferase comprising a feedback-deregulated variant of a Corynebacterium glutamicum ATP phosphoribosyltransferase having at least 70% amino acid sequence identity with SEQ ID NO:7;
- wherein the engineered fungal cell produces histamine at a level at least 75 mg/L of culture medium.
4 more elements
Dependencies are inferred from the claim text.