Sialyltransferase variants having neosialidase activity
AssigneeThe Regents of the University of California
InventorsXi Chen, John B. McArthur, Andrew J. Fisher
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Abstract
α2-6-Sialyltransferase (2,6ST) variants having improved α2-6-specific sialidase activity as compared to the native 2,6ST enzymes are described. The variants include GT80 sialyltransferases such as P. damselae Pd2,6ST. Methods for making de-sialylated products and screening sialidase activity are also described.
Claims7
An α2-6-sialyltransferase (2,6ST) variant having increased α2-6-specific sialidase catalytic efficiency (kcat/KM) as compared to a native 2,6ST, wherein the 2,6ST variant comprises an amino acid sequence having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:2 and comprising one or more of:
- a leucine residue at a position corresponding to residue 232 of SEQ ID NO:
- 1;
- a serine residue at a position corresponding to residue 356 of SEQ ID NO:
- 1;
- and
- a phenylalanine residue at a position corresponding to residue 361 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.