Polysaccharide and uses thereof
AssigneeGlaxosmithkline Biologicals SA
InventorsMichael T. Kowarik, Michael L. Wetter, Stefan J. Kemmler, Micha A. Häuptle, Veronica Gambillara, Manuela Mally
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Abstract
Provided herein is an E. coli O polysaccharide, O25B. Also provided herein are prokaryotic host cells containing enzymes (e.g., glycosyltransferases) used in O25B production. The host cells provided herein produce O25B bioconjugates, wherein said bioconjugates contain O25B linked to a carrier protein. Further provided herein are compositions, e.g., pharmaceutical compositions, including O25B and/or bioconjugates containing O25B. Such compositions can be used as vaccines against infection with ExPEC, and may further include one or more additional bioconjugates.
Claims22
A composition comprising a glycoconjugate of an E. coli O25B antigen covalently coupled to a carrier protein, wherein the E. coli O25B antigen comprises the structure of Formula O25B′:
- wherein n is an integer of 1 to 30.
A composition comprising an E. coli O25B antigen and at least one of the following:
- (i) an E. coli O1 antigen, (ii) an E. coli O2 antigen, and/or (iii) an E. coli O6 antigen;
- wherein at least the E. coli O25B antigen is covalently coupled to a carrier protein.
A composition comprising (i) an E. coli O25B macromolecule, (ii) an E. coli O1 macromolecule, (iii) an E. coli O2 macromolecule, and (iv) an E. coli O6 macromolecule, wherein at least the E. coli O25B macromolecule is conjugated to a carrier protein.
Dependencies are inferred from the claim text.