Methods for biological sample processing and analysis
AssigneeUltima Genomics, Inc.
InventorsGilad Almogy, Kristopher Barbee, Nathan Beckett
Life of this patent
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Abstract
Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.
Claims30
A method for processing a biological analyte, comprising:
- (a) directing a solution comprising a plurality of probes from a first location of a substrate to a second location of said substrate, wherein said first location is different than said second location, wherein said biological analyte is immobilized adjacent to said substrate at said first location or said second location, and wherein said directing is performed in absence of flowing said solution through a flow cell channel;
- (b) coupling at least one probe of said plurality of probes to said biological analyte;
- and
- (c) detecting, by providing an excitation light down to said substrate and using a detector comprising an optical imaging objective disposed above said substrate, wherein said optical imaging objective is at least partially immersed in a fluid in contact with said substrate, one or more signals or signal changes from said biological analyte having said at least one probe coupled thereto.
A method for processing a biological analyte, comprising:
- (a) directing a solution comprising a plurality of probes along one or more flow paths from a first location of a substrate to a second location of said substrate, wherein said first location is different than said second location, wherein said biological analyte is immobilized adjacent to said substrate at said first location or said second location, and wherein said one or more flow paths are not part of a flow cell channel;
- (b) coupling at least one probe of said plurality of probes to said biological analyte;
- and
- (c) detecting, by providing an excitation light down to said substrate and using a detector comprising an optical imaging objective disposed above said substrate, wherein said optical imaging objective is at least partially immersed in a fluid in contact with said substrate, one or more signals or signal changes from said biological analyte having said at least one probe coupled thereto.
Dependencies are inferred from the claim text.