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US 11,732,247 B2Granted
Method of producing lipid
AssigneeKao Corporation
InventorTatsuro Ozaki
Life of this patent
Estimating the term…
Abstract
A method of improving photosynthetic ability of an alga, containing enhancing expression of a transketolase and a fructose-1,6-bisphosphate aldolase.
Claims6
A method of improving productivity of fatty acids or lipids containing the same as components in a Nannochloropsis cell, the method comprising:
- culturing, in culture medium, a Nannochloropsis cell that has been transformed with, and expresses, a gene encoding transketolase and a gene encoding fructose-1,6-bisphosphate aldolase;
- and
- isolating fatty acids or lipids containing the same as components from the cultured product;
- wherein the productivity of the fatty acids or lipids containing the same as components that are isolated from the cultured product is improved as compared to that of a Nannochloropsis host cell that has not been transformed with either gene;
- and,
- wherein the amino acid sequence of the transketolase is that of the following protein (A) or (B), and the amino acid sequence of the fructose-1,6-bisphosphate aldolase is that of the following protein (C) or (D):
- (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO:
- 1;
- (B) a protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence of protein (A), and having transketolase activity;
- (C) a protein consisting of the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (D) a protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence of protein (C), and having fructose-1,6-bisphosphate aldolase activity.
10 more elements
Dependencies are inferred from the claim text.