Attenuated strains of oncolytic rhabdovirus and uses thereof in tumor treatment
AssigneeFantasia Biopharma (Zhejiang) Co. Ltd
InventorsFrank XiaoFeng Qin, Jing Xia
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Abstract
Provided is a modified matrix protein of a vesicular stomatitis virus, wherein the protein has amino acid substitutions at position 21, position 51, position 111 and position 221. Further provided are an attenuated strain of the vesicular stomatitis virus producing the modified matrix protein, a composition containing the attenuated strain, and uses thereof in preparing a drug for killing abnormally proliferating cells, inducing and promoting antitumor immune response, or eliminating immunosuppression in a microenvironment of a tumor tissue.
Claims28
A modified matrix protein (M) of a recombinant oncolytic rhabdovirus, wherein an amino acid sequence encoding the modified matrix protein (M) comprises a sequence which is at least 98% identical to an amino acid sequence as set forth in SEQ ID NO:
- 1;
- and
- wherein the sequence of the modified matrix protein (M) is the amino acid sequence encoding the modified matrix protein (M) and has the following mutations as compared with SEQ ID NO:1:
- (i) mutation of glycine G to glutamic acid E at position 21,
- (ii) mutation of methionine M to alanine A at position 51,
- (iii) mutation of leucine L to phenylalanine F at position 111, and
- (iv) mutation of valine V to phenylalanine F at position 221.
An isolated peptide encoded by an amino acid sequence, wherein the amino acid sequence comprises a sequence which is at least 98% identical to an amino acid sequence of SEQ ID NO:
- 1;
- wherein the amino acid sequence encoding the isolated peptide has the following mutations as compared with SEQ ID NO:1:
- (i) mutation of glycine G to glutamic acid E at position 21,
- (ii) mutation of methionine M to alanine A at position 51,
- (iii) mutation of leucine L to phenylalanine F at position 111, and
- (iv) mutation of valine V to phenylalanine F at position 221.
Dependencies are inferred from the claim text.