Paramyxoviridae expression system
AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsVeljko Nikolin, Alissa Benter, Andreas Gallei
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Abstract
The present invention relates to the field of (vector) vaccines, and especially to an enhanced arrangement of nucleotide sequences for expressing a Paramyxoviridae virus containing an exogenous gene of interest. The present invention further concerns related expression cassettes and vectors, which are suitable to express genes of interest, especially antigen encoding sequences. The viral vectors of the present invention are useful for producing an immunogenic composition or vaccine.
Claims20
A Paramyxoviridae virus vector comprising an expression cassette inserted between the P gene and the M gene, wherein said expression cassette comprises
- a first nucleotide sequence, wherein said first nucleotide sequence is a heterologous or exogenous nucleotide sequence of interest, and
- a second nucleotide sequence flanking the 5′ end of the first nucleotide sequence, wherein said second nucleotide sequence is the 5′ non-coding region of an N gene of a Paramyxoviridae virus, and
- a third nucleotide sequence flanking the 3′ end of the first nucleotide sequence, wherein said third nucleotide sequence comprises or consists of the 3′ non-coding region of an H gene of a Paramyxoviridae virus.
Dependencies are inferred from the claim text.