Lactic acid bacteria, methods and uses thereof
AssigneeBiogaia AB
InventorStefan Roos
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel strains of lactic acid bacteria, more specifically, novel strains of Lactobacillus reuteri, capable of utilizing ethanolamine. This characteristic makes it possible for the bacteria to compete for the same substrate as pathogenic ethanolamine-utilizing pathogens, thereby presenting an effective means to combat infections resulting from such pathogenic bacteria. There is also provided a method of selecting further ethanolamine-utilizing lactic acid bacteria, as well as other methods and uses involving said novel strains and other ethanolamine-utilizing lactic acid bacteria.
Claims18
A method for treating or reducing the risk of developing a condition or disorder caused by or related to an ethanolamine-utilizing pathogen in a subject in need thereof, said method comprising administering to the subject an effective amount of lactic acid bacteria capable of utilizing ethanolamine produced by
- (a) culturing in a culture medium lactic acid bacteria that comprises a gene encoding ethanolamine ammonia lyase large subunit EutB, a gene encoding a microcompartment structural protein EutL, and/or a gene encoding an ethanolamine utilization protein EutH and is capable of utilizing ethanolamine;
- (b) adding to the culture medium of (a) a first amount or concentration of ethanolamine at a first point in time and growing said culture;
- (c) optionally adding to the culture of (b) a second amount or concentration of ethanolamine at a second point in time and growing said culture;
- and
- (d) retrieving the lactic acid bacteria from said culture medium.
A method for treating or reducing the risk of developing a condition or disorder caused by or related to an ethanolamine-utilizing pathogen in a subject in need thereof, the method comprising administering to the subject an effective amount of a lactic acid bacteria that has been induced by ethanolamine during growth, wherein the lactic acid bacteria comprises a gene encoding ethanolamine ammonia lyase large subunit EutB, a gene encoding a microcompartment structural protein EutL, and/or a gene encoding an ethanolamine utilization protein EutH and is capable of utilizing ethanolamine.
A method for treating or reducing the risk of developing a condition or disorder caused by or related to an ethanolamine-utilizing pathogen in a subject in need thereof, said method comprising administering to the subject an effective amount of a Lactobacillus reuteri strain capable of utilizing ethanolamine, the Lactobacillus reuteri strain capable of utilizing ethanolamine produced by
- (a) culturing in a culture medium L. reuteri bacteria that comprises a gene encoding ethanolamine ammonia lyase large subunit EutB, a gene encoding a microcompartment structural protein EutL, and/or a gene encoding an ethanolamine utilization protein EutH, and is capable of utilizing ethanolamine;
- (b) adding to the culture medium of (a) a first amount or concentration of ethanolamine at a first point in time and growing said culture;
- (c) optionally adding to the culture of (b) a second amount or concentration of ethanolamine at a second point in time and growing said culture;
- and
- (d) retrieving the L. reuteri bacteria from said culture medium.
Dependencies are inferred from the claim text.