Sirtuin 4 (SIRT4) lipoamidase activity and uses thereof
AssigneeThe Trustees of Princeton University
InventorsThomas Shenk, Todd M. Greco, Ileana M. Cristea, Rommel A. Mathias, Adam Oberstein
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Abstract
The present application provides a method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human sirtuin 4 (SIRT4) comprising measuring a level of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the cell. The present application also demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.
Claims12
A method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human Sirtuin 4 (SIRT4) comprising measuring relative abundance of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the mammalian cell.
Dependencies are inferred from the claim text.