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US 11,726,026 B2Granted
Fluorescence based extracellular enzyme activity assay for early detection of biofouling in seawater desalination systems
AssigneeKing Abdullah University of Science and Technology
InventorsBabar Khalid Khan, TorOve Leiknes
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Abstract
A method and system for early detection of biofouling utilizing a fluorescence based extracellular enzyme activity assay is disclosed. The method provides a means for early detection of biofouling on membranes used in flow-through membrane filtration systems for with a feed water flow, such as waste water of water for desalination. Also disclosed is an at-line sensor utilizing the fluorescence based extracellular enzyme activity assay that is positioned in the membrane filtration system for detection of biofouling.
Claims19
A method for detecting membrane fouling comprising the steps of:
- providing a membrane fouling sensor positioned adjacent to a feed solution stream in a membrane filtration system, wherein a portion of a feed solution stream flows through the membrane fouling sensor contacting a sensor membrane therein;
- said membrane fouling sensor having an inlet valve directing the feed stream to the sensor membrane and an outlet valve directing the feed stream from the sensor membrane back to the feed solution stream proximal to a main membrane filtration unit;
- allowing the feed solution stream to contact the membrane fouling sensor during the normal operation of the membrane filtration system, such that the sensor membrane is subject to substantially the same conditions as the membrane in the main membrane filtration unit;
- operating the membrane filtration system for a time period ranging from 1 hour to 100 hours, during which time the sensor membrane and the membrane in the main membrane filtration unit are exposed to any microorganisms in the feed solution stream and said microorganisms may adhere to the sensor membrane and the membrane in the main membrane filtration unit causing one or more biofilm to develop on the membranes and producing extracellular enzymes;
- creating a closed system in the membrane fouling sensor by closing the inlet and outlet valves thereby trapping a portion of the feed solution stream and any microbial extracellular enzymes in the membrane fouling sensor;
- injecting a fluorogen-substrate into the membrane fouling sensor via an inject port positioned distal to the inlet valve and proximal to the sensor membrane;
- allowing the fluorogen-substrate to contact the feed solution stream and any microbial extracellular enzymes trapped in the membrane fouling sensor for 1-60 minutes;
- removing a portion of the feed solution stream trapped in the membrane fouling sensor via the flush port;
- using a fluorometer to measure the removed portion of the feed solution stream for fluorescence produced from the fluorogen-substrate interaction with extracellular enzymes;
- correlating the measured fluorescence with extracellular enzyme activity in the sample;
- and
- using the correlation to determine a biofouling level of the membranes.
9 more elements
A membrane fouling sensor comprising:
- a sensor unit positioned adjacent to a main feed solution stream proximal to a main membrane filtration unit in a membrane filtration system, said sensor unit having a filtration membrane;
- an inlet valve positioned at the main feed solution stream that diverts a portion of the main feed solution stream to the sensor unit as a sensor feed stream when opened and prevents the main feed solution stream from entering the sensor unit when closed;
- an outlet valve that directs the sensor feed stream back to the main feed solution stream, distal to the inlet valve and proximal to the main membrane filtration unit when opened, and retains the sensor feed stream in the sensor unit when closed,
- a feed stream conduit positioned between the inlet valve and the outlet valve that directs the main feed solution stream from the inlet valve to the outlet valve through the sensor unit,
- an inject port in the feed stream conduit distal to the inlet valve and proximal to the sensor unit;
- and
- a flush port in the feed stream conduit distal to the sensor unit and proximal to the outlet valve.
4 more elements
A method of detecting biofouling of a membrane comprising the steps of:
- collecting a sample of a permeate from a membrane filtration system, said permeate resulting when a feed stream solution has been filtered by the membrane;
- selecting a fluorogen-substrate comprising Methylumbelliferone (MUF) that can be cleaved by one or more microbial extracellular enzymes;
- mixing the permeate sample with the fluorogen-substrate;
- using a fluorometer to measure fluorescence produced by the interaction of one or more microbial extracellular enzymes in the permeate sample on the fluorogen-substrate;
- correlating the fluorescence of the permeate sample and fluorogen-substrate mixture to determine the level of extracellular enzyme activity in the permeate as an indication of biofouling of the membrane;
- and
- adjusting a pH of the fluorogen-substrate to a range of pH7-pH10 prior to mixing with the permeate sample.
4 more elements
Dependencies are inferred from the claim text.