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US 11,725,184 B2Granted
Culture method for organoids
AssigneeKoninklijke Nederlandse Akademie van Wetenschappen
InventorsMeritxell Huch Ortega, Johannes Carolus Clevers, Sylvia Fernandez Boj
Life of this patent
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Abstract
The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.
Claims15
An expansion medium, comprising a basal medium for animal or human cells to which is added:
- one or more receptor tyrosine kinase ligands, one or more Wnt agonist wherein the Wnt agonist is an Lgr5 agonist, a TGF beta inhibitor, and a cAMP pathway activator,
- wherein the TGF-beta inhibitor is able to reduce the activity of the TGF-beta signaling pathway that acts via Smad2 and/or Smad3.
Dependencies are inferred from the claim text.