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US 11,725,044 B2Granted
Method for producing polyclonal antibodies with improved complement-dependent cytotoxicity
AssigneeXenothera
InventorsOdile Duvaux, Jean-Paul Soulillou
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Abstract
The present invention relates to polyclonal antibodies directed against at least one non-human biological pathogen, or against at least one molecule derived from said pathogen, towards a human or a non-human animal organism, wherein the said polyclonal antibodies are devoid of an antigenic determinant selected in a group comprising (i) N-glycolneuraminic acid (Neu5Gc) and/or (ii) a-1,3-galactose, and their use as a medicament.
Claims2
A method for producing polyclonal antibodies, comprising the steps of:
- a) providing a genetically altered pig lacking:
- (i) a gene encoding a functional cytidine-5′-monophosphate N-acetyl neuraminic acid hydrolase (CMAH) and (ii) a gene encoding a functional α-(1,3)-galactosyltransferase;
- b) immunizing said genetically altered pig against at least one non human biological pathogen(s) towards a human, or against at least one peptidoglycan derived from said pathogen(s);
- and
- c) collecting the antibodies having improved Complement Dependent Cytotoxicity (CDC) activity contained in a body fluid of said genetically altered pig of step b),
- said biological pathogen, or the peptidoglycan derived from said pathogen, towards a human, being a bacteria or a virus,
- wherein the polyclonal antibodies having an improved CDC activity, as compared to:
- polyclonal antibodies obtained from the same method from a wildtype pig, wherein said wildtype pig comprises (i) a gene encoding a functional cytidine-5′-monophosphate N-acetyl neuraminic acid hydrolase (CMAH) and (ii) a gene encoding a functional α-(1,3)-galactosyltransferase (WT CMAH+/GGTA1+ pig), and
- polyclonal antibodies obtained through the same method from a pig which comprises only one among (i) a gene encoding a functional cytidine-5′-monophosphate N-acetyl neuraminic acid hydrolase (CMAH) (CMAH−/GGTA1+ pig) or (ii) a gene encoding a functional α-(1,3)-galactosyltransferase (CMAH+/GGTA1− pig).
6 more elements
Dependencies are inferred from the claim text.