Immunoassay method to prevent inhibition of antigen-antibody binding interactions in mucosal fluids

Abstract

The purpose of the present invention is to: provide an agent that effectively suppresses inhibition of antigen-antibody reaction in an immunoassay using a sample containing a body fluid, in particular, a component derived from a biological mucosal membrane, such as saliva; and to suppress false positive and false negative results in the immunoassay. The present invention provides an agent for suppressing inhibition of immune reaction, characterized in that the agent comprises a compound of the following (1) or (2): (1) Sulfonic acid compound of the formula R1—SO3H or a salt thereof. (In the formula, R1 is selected from the group consisting of: a straight-chain C5-C30 alkyl group; a straight-chain C1-C30 alkyl group substituted with an aryl group having at least one straight-chain C5-C30 alkyl group; and an aryl group having at least one straight-chain C5-C30 alkyl group. These groups may include a substituent group); and (2) Quaternary ammonium ion of the formula N+—R2R3R4R5 or a salt thereof. (In the formula, R2—R5 are each independently a straight-chain C1-C30 alkyl group, or an aryl group substituted with at least one straight-chain C5-C30 alkyl group. These groups may include a substituent group); wherein the agent is capable of suppressing immune reaction inhibitory action caused by a body fluid in an immunoassay sample.

Dependencies are inferred from the claim text.