Method for processing tissue samples
AssigneeS2 Genomics, Inc.
InventorsStevan Bogdan Jovanovich, Frank Zaugg, Kaiwan Chear, Roger McIntosh, Nathan Pereira
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Abstract
This disclosure provides methods for producing a sample of subcellular organelles, particularly nuclei, from a tissue. In some embodiments, this disclosure provides a method of processing a tissue sample involves performing enzymatic/chemical disruption of tissue in a chamber to produce disrupted tissue comprising released cells and/or nuclei and debris; separating the released cells and/or nuclei from the debris therein; and moving the released cells and/or nuclei. In some instances, the method comprises mechanical disruption of the tissue sample.
Claims39
A method of preparing a sample, comprising:
- (a) introducing a tissue or cell sample into a chamber such that the tissue or cell sample is in a liquid medium that is in contact with a non-porous grinding stator within the chamber;
- (b) introducing a tissue disruptor comprising disruptor grinding features that protrude from a surface of the tissue disruptor into the chamber;
- (c) performing mechanical disruption of the tissue or cell sample in the chamber, wherein the mechanical disruption is conducted by grinding the tissue or cell sample between the disruptor grinding features and the grinding stator to produce (i) released cells or released subcellular organelles and (ii) debris;
- (d) separating the released cells or the released subcellular organelles from a portion of the debris;
- and
- (e) collecting the released cells or released subcellular organelles in a liquid suspension.
A method of analyzing cells comprising:
- (a) introducing a tissue or cell sample into a chamber such that the tissue or cell sample is in a liquid medium that is in contact with a grinding stator within the chamber;
- (b) introducing a tissue disruptor comprising disruptor grinding features that protrude from a surface of the tissue disruptor into the chamber, wherein:
- i. the tissue disruptor comprises a portion configured as a disk or cylinder that fits within the chamber;
- ii. the chamber comprises a space between the portion of the tissue disruptor configured as a disk or cylinder and a wall of the chamber, and
- iii. the chamber further comprises a port positioned above the disruptor grinding features when the tissue disruptor is fully depressed;
- (c) performing mechanical disruption of the tissue or cell sample in the chamber, wherein the mechanical disruption is conducted by grinding the tissue or cell sample between the disruptor grinding features and the grinding stator to produce (i) released cells or released subcellular organelles and (ii) debris;
- (d) separating the released cells or the released subcellular organelles from a portion of the debris;
- and
- (e) collecting the released cells or released subcellular organelles in a liquid suspension.
Dependencies are inferred from the claim text.