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US 11,718,857 B2Granted
Broad host range genetic tools for engineering microalgae
AssigneeAlliance for Sustainable Energy, LLC
InventorsMichael T. Guarnieri, Jeffrey George Linger, Lukas Royce Dahlin
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Abstract
Disclosed herein are a suite of genetic tools suitable for engineering both the nucleus and chloroplast in diverse microalgae.
Claims8
A method for transforming a Picochlorum renovo algal chloroplast, the method comprising:
- introducing an exogenous selection marker into the Picochlorum renovo algal chloroplast;
- and
- bombarding a culture of the Picochlorum renovo algal chloroplast with a loaded particle.
A method for transforming a Picochlorum renovo algal nucleus, the method comprising:
- synthesizing a nuclear integration cassette;
- integrating the nuclear integration cassette into a plasmid backbone;
- and
- codon optimizing a gene encoding a selection marker and a gene encoding a peptide into a genome of the Picochlorum renovo algal nucleus;
- bombarding a culture of the Picochlorum renovo algal nucleus with a loaded particle.
2 more elements
Dependencies are inferred from the claim text.