Back to results
US 11,718,854 B2Granted
Gene targets for improved enzyme production in fungi
AssigneesBattelle Memorial Institute, National Technology & Engineering Solutions of Sandia, LLC
InventorsScott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
Life of this patent
Estimating the term…
Abstract
Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
Claims32
An isolated Aspergillus fungus, comprising:
- a genetic inactivation of a mstC gene encoding a polypeptide comprising monosaccharide transporter activity and an amino acid sequence with at least 80% sequence identity to the amino acid sequence of SEQ ID NO:
- 2, and
- a polynucleotide comprising a glucoamylase promoter operably linked to a heterologous protein coding sequence encoding a heterologous protein,
- wherein said heterologous protein coding sequence is heterologous to the isolated Aspergillus fungus, and wherein said heterologous protein coding sequence is heterologous or homologous to the glucoamylase promoter.
1 more element
An isolated Neurospora fungus, comprising:
- a genetic inactivation of a NCU01633 gene encoding a polypeptide comprising monosaccharide transporter activity and an amino acid sequence with at least 80% sequence identity to the amino acid sequence of SEQ ID NO:
- 11, and
- a polynucleotide comprising a glucoamylase promoter operably linked to a heterologous protein coding sequence encoding a heterologous protein,
- wherein said heterologous protein coding sequence is heterologous to the isolated Neurospora fungus, and wherein said heterologous protein coding sequence is heterologous to or homologous to the glucoamylase promoter.
1 more element
Dependencies are inferred from the claim text.