Method for visualizing neurons
AssigneeYeu-Kuang Hwu
InventorsYeu-Kuang Hwu, Shin-Tai Chen, Shun-Min Yang
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Abstract
A method for visualizing neurons is provided. The method comprises a staining step and a visualizing step. The staining step comprises following steps: placing a neural tissue sample in an acrolein solution in the dark for fixation; placing the fixed neural tissue sample in a Golgi-Cox solution in the dark; replacing the Golgi-Cox solution; incubating the neural tissue sample placed in the replaced Golgi-Cox solution at a range of 36° C. to 38° C.; gradiently dehydrating the neural tissue sample; and embedding the dehydrated neural tissue sample with Petropoxy 154 resin. The visualizing step comprises: performing data acquisition and image reconstruction on the neural tissue sample using X-ray microscopy.
Claims17
A method for visualizing neurons, comprising:
- a staining step, comprising:
- placing a neural tissue sample in an acrolein solution in the dark for fixation;
- placing the fixed neural tissue sample in a Golgi-Cox solution in the dark;
- replacing the Golgi-Cox solution;
- incubating the neural tissue sample placed in the replaced Golgi-Cox solution at a temperature ranging from 36° C. to 38° C.;
- gradiently dehydrating the neural tissue sample;
- embedding the dehydrated neural tissue sample with Petropoxy 154 resin, wherein the Petropoxy 154 resin is composed of 90% of Petropoxy 154 resin and 10% of 99.5% ethanol;
- and
- a visualizing step, comprising:
- visualizing and image-taking the neural tissue sample using X-ray microscopy.
Dependencies are inferred from the claim text.