Microbiota sequence variants of tumor-related antigenic epitopes

A method for identification of a human microbiota peptide sequence variant of a human tumor-related antigenic epitope sequence, the method comprising the following steps:

• (i) selection of a human tumor-related antigen of interest,
• (ii) identification of at least one epitope comprised in the human tumor-related antigen selected in step (i) and determination of its sequence, and
• (iii) identification of at least one human microbiota peptide sequence variant of the epitope sequence identified in step (ii),
• wherein the method further comprises the following step:
• (iv) testing binding of the at least one human microbiota peptide sequence variant to MHC molecules and obtaining a binding affinity,
• testing binding of the respective human tumor-related antigenic epitope to WIC molecules and obtaining a binding affinity;
• and
• comparing of the binding affinities obtained for the human microbiota peptide sequence variant and for the respective human tumor-related antigenic epitope and selecting human microbiota peptide sequence variants having a higher binding affinity to MHC than their respective human tumor-related antigenic epitopes.