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US 11,708,596 B2Granted
Analyzing and using motility kinematics of microorganisms
AssigneePhast Corp.
InventorsKwangmin Son, Roman Stocker
Life of this patent
Estimating the term…
Abstract
Among other things, motility of at least one individual microorganism or a change in motility of at least one individual microorganism or both is or are characterized. The characterized motility or change in motility is used to detect the presence or count of the at least one individual microorganism, or determine the identity of a species or strain of the at least one individual microorganism, or determine a susceptibility of the at least one individual microorganism to one or more antibiotics or other antimicrobials.
Claims25
A method comprising:
- (a) subjecting a first portion and a second portion of a bodily sample of a human or an animal to different known concentrations of one or more antibiotics, the first portion and the second portion each containing one or more individual bacteria or fungi of one or more strains;
- (b) by an imaging device, capturing one or more images of the first portion and one or more images of the second portion;
- (c) by computer, measuring physical trajectories of the one or more individual bacteria or fungi from the one or more captured images of the sample first portion;
- (d) by computer, generating motility kinematics data corresponding to the measured physical trajectories for at least one of the one or more individual bacteria or fungi of one or more strains in the first portion;
- (e) by computer, measuring physical trajectories from the one or more captured images of the second portion;
- (f) by computer, generating motility kinematics data corresponding to the measured physical trajectories for at least one of the one or more individual bacteria or fungi of one or more strains in the second portion;
- (g) by computer, determining from the motility kinematics data a first physical motility of the at least one of the one or more individual bacteria or fungi of one or more strains in the first portion and a second first physical motility of the at least of the one or more individual bacteria or fungi of one or more strains in the second portion;
- (h) by computer, comparing the first determined physical motility and the second determined physical motility and generating a magnitude of a difference between the first determined physical motility and the second determined physical motility;
- and
- (i) by computer, identifying one or more strains of bacteria or fungi from the magnitude of the difference between the first determined physical motility and the second determined physical motility and from stored data indicative of relationships of physical motility of bacteria or fungi to identify one or more strains of bacteria or fungi subjected to the one or more known concentrations of antibiotics.
7 more elements
A method comprising:
- (a) subjecting a first portion and a second portion of a bodily sample of a human or an animal to different known concentrations of one or more antibiotics, the first portion and the second portion each containing one or more individual bacteria or fungi of one or more strains having a movement in the sample, the movement resulting from an external influence other than the one or more antibiotics;
- (b) by an imaging device, capturing in at least two different contexts one or more images of the first portion and one or more images of the second portion;
- (c) by computer, measuring physical trajectories from the one or more captured images of the first portion corresponding to the movement of the one or more individual bacteria or fungi of one or more strains resulting from the external influence;
- (d) by computer, generating motility kinematics data corresponding to the measured physical trajectories for at least one of the one or more individual bacteria or fungi of one or more strains in the first portion;
- (e) by computer, measuring physical trajectories from the one or more captured images of the second portion corresponding to the movement of the one or more individual bacteria or fungi of one or more strains resulting from the external influence;
- (f) by computer, generating motility kinematics data corresponding to the measured physical trajectories for at least one of the one or more individual bacteria or fungi of one or more strains in the second portion;
- (g) by computer, determining from the motility kinematics data a first physical motility of the at least one of the one or more individual bacteria or fungi of one or more strains in the first portion and a second first physical motility of the at least of the one or more individual bacteria or fungi of one or more strains in the second portion;
- (h) by computer, comparing the first determined physical motility and the second determined physical motility and generating a magnitude of a difference between the first determined physical motility and the second determined physical motility;
- and
- (i) by computer, identifying one or more strains of bacteria or fungi from the magnitude of the difference between the first determined physical motility and the second determined physical motility and from stored data indicative of relationships of physical motility of bacteria or fungi to identify one or more strains of bacteria or fungi subjected to the one or more known concentrations of antibiotics.
7 more elements
Dependencies are inferred from the claim text.