Synthetic metabolic funneling for biochemical production
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsDavid Nielsen, Brian Thompson
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Abstract
Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.
Claims15
A method for preparing a biochemical product that is muconic acid, the method comprising:
- i) contacting a recombinant host cell with a fermentable carbon source, wherein the recombinant host cell is a bacteria, yeast, or filamentous fungi cell, wherein the recombinant host cell co-expresses a recombinant MA1 pathway and a recombinant MA5 pathways, and wherein:
- a) each pathway is capable of producing the same final biochemical product;
- b) each pathway comprises at least one gene encoding a polypeptide;
- c) each pathway is derived from a different endogenous metabolite as its immediate precursor;
- and
- d) each pathway converges to the same final product or the same intermediate metabolite;
- and
- ii) growing said recombinant host cell for a time sufficient to synthesize the product;
- wherein the recombinant MA1 pathway comprises at least one gene encoding a polypeptide having 3-dehydroshikimate dehydratase activity;
- at least one gene encoding a polypeptide having protocatechuate decarboxylase activity;
- and at least one gene encoding a polypeptide having 1,2-catechol dioxygenase activity;
- and
- wherein the recombinant MA5 pathway comprises at least one gene encoding a polypeptide having chorismate lyase activity;
- at least one gene encoding a polypeptide having p-hydroxybenzoate hydroxylase activity;
- at least one gene encoding a polypeptide having protocatechuate decarboxylase activity;
- and at least one gene encoding a polypeptide having 1,2-catechol dioxygenase activity.
Dependencies are inferred from the claim text.