Polypeptide and nucleic acid capable of changing amylose content (ac) in plant, and use thereof
AssigneeShandong Shunfeng Biotechnology Co., Ltd.
InventorsJinshan Zhang, Xiaorui Huang, Xiaomu Niu
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Abstract
A mutant granule-bound starch synthase 1 (GBSS1) polypeptide and a nucleic acid, and use thereof are provided. Compared to an amino acid sequence of a parent GBSS1, the mutant GBSS1 polypeptide has a mutation at an amino acid corresponding to amino acid 427 and/or amino acid 428 of an amino acid sequence shown in SEQ ID NO: 1. An amylose content (AC) in a plant changes after the plant undergoes GBSS1 mutation, which has very promising application prospects in the improvement of edible quality of rice.
Claims9
A polynucleotide encoding a mutant GBSS1 polypeptide capable of increasing an amylose content (AC) in a plant, wherein the polynucleotide comprises a nucleotide sequence having at least 80% identity with SEQ ID NO:
- 16, SEQ ID NO:
- 17, or SEQ ID NO:
- 18, and wherein the mutant GBSS1 polypeptide comprises an amino acid sequence having at least 80% identity with SEQ ID NO:
- 1 and further comprises a mutation(s) at amino acid residue 427 and/or 428 compared to the amino acid sequence shown in SEQ ID NO:
- 1, wherein the amino acid residue 427 is mutated into arginine (R) and/or the amino acid residue 428 is mutated into glycine (G).
A host cell, the host cell comprising:
- a mutant granule-bound starch synthase 1_(GBSS1) polypeptide capable of increasing an amylose content (AC) in a plant, the mutant GBSS1 polypeptide comprising an amino acid sequence at least 80% identity with SEQ ID NO:
- 1, further comprising a mutation(s) at amino acid residue 427 and/or 428 compared to the amino acid sequence shown in SEQ ID NO:
- 1, wherein the amino acid residue 427 is mutated into arginine (R) and/or the amino acid residue 428 is mutated into glycine (G),
- a polynucleotide encoding a mutant GBSS1 polypeptide capable of increasing an amylose content (AC) in a plant, wherein the polynucleotide comprises a nucleotide sequence having at least 80% identity with SEQ ID NO:
- 16, SEQ ID NO:
- 17, or SEQ ID NO:
- 18, and wherein the mutant GBSS1 polypeptide comprises an amino acid sequence having at least 80% identity with SEQ ID NO:
- 1 and further comprises a mutation(s) at amino acid residue 427 and/or 428 compared to the amino acid sequence shown in SEQ ID NO:
- 1, wherein the amino acid residue 427 is mutated into arginine (R) and/or the amino acid residue 428 is mutated into glycine (G), or
- a nucleic acid construct comprising a polynucleotide encoding the mutant GBSS1 polypeptide capable of increasing an AC in a plant, wherein the polynucleotide comprises a nucleotide sequence having at least 80% identity with SEQ ID NO:
- 16, SEQ ID NO:
- 17, or SEQ ID NO:
- 18, and wherein the mutant GBSS1 polypeptide comprising an amino acid sequence having at least 80% identity with SEQ ID NO:
- 1 and further comprising a mutation(s) at amino acid residue 427 and/or 428 compared to the amino acid sequence shown in SEQ ID NO:
- 1.wherein the amino acid residue 427 is mutated into arginine (R) and/or the amino acid residue 428 is mutated into glycine (G), and a regulatory element operably linked to the polynucleotide encoding the mutant GBSS1 polypeptide, wherein the regulatory element is one or more selected from the group consisting of an enhancer, a transposon, a promoter, a terminator, a leader sequence, a polynucleotide sequence, and a marker gene.
A method for preparing a plant with a increased (AC), comprising a step of expressing a mutant granule-bound starch synthase 1 (GBSS1) polypeptide in a plant cell, a plant seed, a plant tissue, a plant part, or the plant, wherein the mutant GBSS1 polypeptide is capable of increasing AC, the mutant GBSS1 polypeptide comprises an amino acid sequence having at least 80% identity with SEQ ID NO:
- 1, and wherein the amino acid residue 427 is mutated into arginine (R) and/or the amino acid residue 428 is mutated into glycine (G) compared to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.