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US 11,708,564 B2Granted
Microorganism expressing active D-proline reductase and method of producing active D-proline reductase
AssigneeCj Cheiljedang Corporation
InventorsJinseung Park, Bo Seong Park, Young Lyeol Yang, In Seok Oh, Nahum Lee, Jun Ok Moon
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Abstract
The present disclosure relates to a microorganism expressing active D-proline reductase.
Claims16
A microorganism comprising:
- (i) a first expression vector encoding PrdA protein and a second expression vector encoding PrdH protein, or
- (ii) a third expression vector encoding PrdH protein and PrdA protein, and a fourth expression vector encoding PrdB protein,
- wherein the PrdH protein comprises a polypeptide having a sequence identity of at least 90% to the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 23, the PrdA protein comprises a polypeptide having a sequence identity of at least 80% to SEQ ID NO:
- 3 or SEQ ID NO:
- 19, and the PrdB protein comprises a polypeptide having a sequence identity of at least 80% to SEQ ID NO:
- 5 or SEQ ID NO:
- 21.
6 more elements
A method of producing a microorganism expressing active D-proline reductase, the method comprising:
- transforming a host cell with a first expression cassette comprising prdA gene or prdA and prdH genes;
- and
- transforming the host cell with a second expression cassette comprising prdB gene or prdB and prdH genes,
- wherein the prdH gene encodes PrdH protein comprising a polypeptide having a sequence identity of at least 90% to SEQ ID NO:
- 1 or SEQ ID NO:
- 23, the prdA gene encodes PrdA protein comprising a polypeptide having a sequence identity of at least 80% to SEQ ID NO:
- 3 or SEQ ID NO:
- 19, and the prdB encodes PrdB protein comprising a polypeptide having a sequence identity of at least 80% to SEQ ID NO:
- 5 or SEQ ID NO:
- 21;
- wherein the microorganism is a microorganism of the genus Escherichia.
8 more elements
Dependencies are inferred from the claim text.