Method for producing artificial recombinant rotavirus
AssigneeOsaka University
InventorsTakeshi Kobayashi, Yuta Kanai
Life of this patent
Estimating the term…
Abstract
Provided is a method for producing an artificial recombinant virus of the family Reoviridae, the method comprising the steps of: (1) introducing a FAST protein expression vector and/or a capping enzyme expression vector into host cells; (2) introducing a vector containing expression cassettes for individual RNA genome segments of a virus or introducing a set of single-stranded RNA transcripts from the expression cassettes into host cells; and (3) culturing the host cells. The method of the present invention allows more efficient production of an artificial recombinant virus of the family Reoviridae as compared with conventional methods and allows artificial recombinant rotavirus production without using a helper virus.
Claims11
A method for producing an artificial recombinant rotavirus, comprising:
- introducing a vector containing expression cassettes for 11 individual RNA genome segments of a rotavirus or introducing a set of 11 single-stranded RNA transcripts from the expression cassettes into host cells,
- overexpressing a rotavirus NSP2 gene product or a rotavirus NSP5 gene product in the host cells, and
- culturing the host cells.
A method for producing an artificial recombinant rotavirus, comprising:
- introducing a vector containing expression cassettes for 11 individual RNA genome segments of a rotavirus or introducing a set of 11 single-stranded RNA transcripts from the expression cassettes into host cells, which express a FAST protein, and
- culturing the host cells.
A method for producing an artificial recombinant rotavirus, comprising:
- introducing a vector containing expression cassettes for 11 individual RNA genome segments of a rotavirus or introducing a set of 11 single-stranded RNA transcripts from the expression cassettes into host cells, which express a capping enzyme, and
- culturing the host cells.
Dependencies are inferred from the claim text.