Recombinant rhabdovirus encoding for CCL21
AssigneesBoehringer Ingelheim International GmbH, Viratherapeutics GmbH
InventorsPhilipp Mueller, Klaus Erb, Patrik Erlmann, Tobias Friederike Nolden, John Edward Park, Guido Wollmann
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Abstract
The present invention relates to the field of oncolytic viruses and in particular to a recombinant rhabdovirus, such as vesicular stomatitis virus encoding in its genome for a CCL21 protein. The invention is further directed to the use of the recombinant virus in the treatment of cancer, and also to methods for producing such viruses.
Claims34
A recombinant vesicular stomatitis virus encoding in its genome at least one human CCL21 protein having a c-terminal truncation wherein said truncated CCL21 protein comprises the amino acids 1-79, 1-81, 1-88, or 1-91, as shown in SEQ ID NO:2 and having at least 80% identity to SEQ ID NO:3, and wherein the gene coding for the glycoprotein G of the recombinant vesicular stomatitis virus is replaced by the gene coding for the glycoprotein GP of Lymphocyte choriomeningitis virus (LCMV), and/or the glycoprotein G is replaced by the glycoprotein GP of LCMV.
A recombinant vesicular stomatitis virus encoding in its genome a vesicular stomatitis virus nucleoprotein (N), large protein (L), phosphoprotein (P), matrix protein (M), glycoprotein (G) and at least one human truncated CCL21 protein comprising the amino acids 1-79, 1-81, 1-88, or 1-91, as shown in SEQ ID NO:2 and having at least 80% identity to SEQ ID NO:3, and further comprising a signal sequence operably linked to said truncated CCL21 protein,
- wherein the gene coding for the glycoprotein G of the vesicular stomatitis virus is replaced by the gene coding for the glycoprotein GP of lymphocyte choriomeningitis virus (LCMV), and/or the glycoprotein G is replaced by the glycoprotein GP of LCMV, and wherein
- the nucleoprotein (N) comprises an amino acid as set forth in SEQ ID NO:7 or a at least 98% identical to SEQ ID NO:7
- wherein the phosphoprotein (P) comprises an amino acid as set forth in SEQ ID NO:8 or at least 98% identical to SEQ ID NO:8
- wherein the large protein (L) comprises an amino acid as set forth in SEQ ID NO:9 or at least 98% identical to SEQ ID NO:9
- the matrix protein (M) comprises an amino acid as set forth in SEQ ID NO:10 or at least 98% identical to SEQ ID NO:10.
A recombinant rhabdovirus encoding in its RNA genome at least one human CCL21 protein, wherein the RNA genome of the recombinant rhabdovirus comprises or consists of a coding sequence identical or at least 75%, identical to SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.