Method and device for acoustically mediated intracellular delivery
AssigneeRoyal Melbourne Institute of Technology
InventorsShwathy Ramesan, Amgad Rezk, Leslie Yeo
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Abstract
An intracellular delivery device (1) including, a piezoelectric substrate (3) having a working surface (8), at least one interdigitated transducer (5) located on and in contact with the working surface (8), and a receptacle (11) located on the working surface for accommodating cells to be targeted for intracellular delivery therein, wherein an alternating signal applied to the interdigitated transducer generates acoustic wave energy through the piezoelectric substrate that can be transferred to the accommodated cells.
Claims20
An intracellular delivery device including, a piezoelectric substrate having a working surface, at least one interdigitated transducer located on and in contact with the working surface, and a receptacle located on the working surface for accommodating a cell media;
- the cell media comprising a plurality of cells to be targeted for intracellular delivery;
- and
- therapeutic molecules or nanoparticles to be transferred by intracellular delivery into the cells;
- wherein an alternating signal applied to the interdigitated transducer generates acoustic wave energy through the piezoelectric substrate that can be transferred to the plurality of cells at a frequency which avoids sonoporation involving membrane disrupting cavitation events near the plurality of cells.
An intracellular delivery method comprising:
- exposing a cell media to acoustic wave energy including surface acoustic waves, the cell media comprising a plurality of cells and therapeutic molecules and/or nanoparticles;
- wherein the therapeutic molecules and/or nanoparticles are transferred into the exposed cells by intracellular delivery,
- further wherein the acoustic wave energy including surface acoustic waves is at a frequency which avoids sonoporation involving membrane disrupting cavitation events near the plurality of cells.
Dependencies are inferred from the claim text.