Antigen receptors and uses thereof

A method for producing a cell expressing an antigen receptor which receptor comprises a first peptide chain and a second peptide chain, the method comprising:

• (A)
• (a) providing a cell;
• (b) providing a first genetic construct, wherein the first genetic construct encodes the first peptide chain comprising
• an immunoglobulin heavy chain variable domain (VH), an immunoglobulin light chain variable domain (VL), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain;
• (c) providing a second genetic construct, wherein the second genetic construct encodes the second peptide chain comprising an immunoglobulin heavy chain variable domain (VH), an immunoglobulin light chain variable domain (VL), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain;
• (d) introducing the first and second genetic constructs into the cell;
• and
• (e) allowing the constructs to be expressed in the cell,
• wherein the VH domain from the first peptide chain is able to form together with the VL domain from the second peptide chain a first antigen binding site, and wherein the VL domain from the first peptide chain is able to form together with the VH domain from the second peptide chain a second antigen binding site;
• or
• (B)
• (a) providing a cell;
• (b) providing a first genetic construct, wherein the first genetic construct encodes the first peptide chain comprising
• two immunoglobulin light chain variable domains (VL), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain;
• (c) providing a second genetic construct, wherein
• the second genetic construct encodes the second peptide chain comprising two immunoglobulin heavy chain variable domains (VH), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain;
• (d) introducing the first and second genetic constructs into the cell;
• and
• (e) allowing the constructs to be expressed in the cell;
• wherein a first VL domain from the first peptide chain is able to form a first antigen binding site together with a first VH domain from the second peptide chain, and
• wherein a second VL domain from the first peptide chain is able to form a second antigen binding site together with a second VH domain from the second peptide chain.

A combination of nucleic acids wherein the first nucleic acid encodes a first peptide chain and a second nucleic acid encodes a second peptide chain, or a nucleic acid encoding both a first and a second peptide chain, wherein

• (i) the first peptide chain comprises an immunoglobulin heavy chain variable domain (VH), an immunoglobulin light chain variable domain (VL), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain, and
• the second peptide chain comprises an immunoglobulin heavy chain variable domain (VH), an immunoglobulin light chain variable domain (VL), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain, and
• wherein the VH domain from the first peptide chain forms together with the VL domain from the second peptide chain a first antigen binding site, and
• wherein the VL domain from the first peptide chain forms together with the VH domain from the second peptide chain a second antigen binding site;
• or
• (ii) the first peptide chain comprises two immunoglobulin light chain variable domains (VL), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain, and
• the second peptide chain comprises two immunoglobulin heavy chain variable domains (VH), a variable region of a T cell receptor chain, and an immunoreceptor signal transmission domain;
• and
• wherein a first VL domain from the first peptide chain forms a first antigen binding site together with a first VH domain from the second peptide chain, and
• wherein a second VL domain from the first peptide chain is able to form a second antigen binding site together with a second VH domain from the second peptide chain.