Methods for producing collagen
AssigneeAvicenna Nutraceutical, LLC
InventorsAli Elnajjar, Ali Mourad, Mark Ernst Brandt, Christopher Lippelt
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Abstract
Disclosed herein are methods for efficiently isolating collagen form a collagen source. The methods are inexpensive and do not require the use of proteolytic enzymes, decolorizing agents, antibacterial and antifungal agents, and the like. Further, the collagen produced by the methods described herein is substantially free of odor and discoloration. Still further, the collagen produced by the methods described herein is suitable to be used in cosmetics, food products, and pharmaceuticals or nutritional supplements.
Claims38
A method for isolating collagen from a collagen source, the method comprising
- (a) contacting the collagen source with an aqueous base to produce a first composition comprising a first supernatant and a first precipitate, wherein the aqueous base comprises an alkali base or an alkaline earth metal base, wherein the alkali base or the alkaline earth metal base has a concentration of 0.05M to 0.5M;
- (b) separating the first precipitate from the first supernatant;
- (c) contacting the first precipitate with a digestive enzyme at a temperature greater than or equal to 25° C. to produce a second composition comprising a second supernatant and a second precipitate, wherein the digestive enzyme comprises protease, papain, bromelain, chymotrypsin, rennin, elastase, pancreatic amylase, pancreatic lipase, pepsin, trypsin, or any combination thereof;
- (d) separating the second supernatant from the second precipitate;
- (e) deactivating the digestion enzyme in the second supernatant comprising adjusting the pH of the second supernatant to a pH greater than 7.0 but less than or equal to 11 to produce a third composition;
- (f) precipitating the collagen from the third composition;
- and
- (g) separating the precipitated collagen from the third composition.
A method for isolating collagen from chicken sternum, the method comprising
- (a) contacting the chicken sternum with aqueous sodium hydroxide to produce a first composition comprising a first supernatant and a first precipitate, wherein the ratio of chicken sternum per mole of sodium hydroxide is from 500 g chicken sternum/mole of base to 5,000 g chicken sternum/mole of base;
- (b) separating the first precipitate from the first supernatant;
- (c) contacting the first precipitate with a digestive enzyme and an aqueous acid at a temperature of greater than or equal to 25° C. to produce a second composition comprising a second supernatant and a second precipitate, wherein the digestive enzyme is pepsin in the amount of 1% to 10% w/w per the collagen source and the aqueous acid is acetic acid having a concentration of from 0.1 M to 2 M;
- (d) separating the second supernatant from the second precipitate;
- (e) deactivating the digestion enzyme in the second supernatant comprising adjusting the pH of the second supernatant to a pH greater than 7.0 but less than or equal to 11 to produce a third composition;
- (f) precipitating the collagen from the third composition comprising (1) adjusting the pH of the third composition to a pH of less than 5.0 by adding an acid to the third composition and (2) adding a salt to the third composition;
- and
- (g) separating the collagen from the third composition.
Dependencies are inferred from the claim text.