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US 11,701,405 B2Granted
Chimeric antigen receptors and methods for reducing toxicity
AssigneeCity of Hope
InventorsXiuli Wang, Stephen J. Forman, Lawrence Stern, Christine E. Brown, Joseph Cohen
Life of this patent
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Abstract
Nucleic acid molecules that include a nucleotide sequence encoding a chimeric antigen receptor (CAR) and a nucleotide sequence encoding a protease sensitive scFv, wherein the chimeric antigen receptor comprises: an scFv targeting a tumor antigen, a spacer, a transmembrane domain, a co-stimulatory domain, and a CD3ζ signaling domain; and the protease-sensitive scFv and the scFv target the same tumor antigen are described.
Claims7
A nucleic acid molecule comprising:
- (a) a nucleotide sequence encoding a chimeric antigen receptor (CAR) comprising amino acids 23 to 665 of SEQ ID NO:
- 31 and (b) a nucleotide sequence encoding a protease sensitive scFv comprising, from amino to carboxy terminus, a VH domain comprising SEQ ID NO:
- 33 and a VL domain SEQ ID NO:34 joined by a protease-sensitive linker that is sensitive to MMP-2 or MMP-9.
Dependencies are inferred from the claim text.