Image acquisition device and image acquisition method

A device comprising:

• a stage configured to hold a sample;
• a light source configured to emit instantaneous light;
• an objective lens disposed to face the sample on the stage;
• a driver configured to move the sample in a scanning direction intersecting an optical axis of the objective lens at a predetermined speed;
• a computer configured to set a plurality of lanes in an image acquisition region of the sample;
• and
• an image sensor configured to capture an image of the sample in respective lanes by lane scanning and to acquire a plurality of tile images;
• wherein the computer is configured to determine whether or not, the lane scanning with respect to the lane that acquired the tile images is re-executed, and wherein the computer is configured to re-execute the lane scanning based on a determination result.