Methods for detecting AAV
AssigneeGenzyme Corporation
InventorsXiaoying Jin, Catherine O'Riordan, Lin Liu, Kate Zhang
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Abstract
Provided herein are methods for determining the serotype of a virus particle and/or or determining the heterogeneity of a virus particle (e.g., an AAV particle). In other embodiments, the invention provides methods to determine the heterogeneity of AAV particles. In some aspects, the invention provides viral particles (e.g., rAAV particles) with improved stability and/or improved transduction efficiency by increasing the acetylation and/or deamidation of capsid proteins.
Claims20
A method to determine the serotype of an adeno-associated virus (AAV) particle comprising
- a) denaturing the AAV particle,
- b) directly subjecting the denatured AAV particle to liquid chromatography/mass spectrometry (LC/MS) intact protein analysis, and
- c) determining the masses of VP1, VP2 and VP3 of the AAV particle;
- wherein the specific combination of masses of VP1, VP2 and VP3 are indicative of the AAV serotype,
- and wherein the method is performed in the absence of a gel separation step.
A method to determine the serotype of a viral particle comprising
- a) denaturing the viral particle,
- b) directly subjecting the denatured viral particle to liquid chromatography/mass spectrometry (LC/MS) intact protein analysis, and
- c) determining the masses of one or more capsid proteins of the viral particle;
- wherein the specific combination of masses of the one or more capsid proteins are indicative of the virus serotype,
- and wherein the method is performed in the absence of a gel separation step.
Dependencies are inferred from the claim text.