Genetically encoded biosensors
AssigneeHoward Hughes Medical Institute
InventorsJonathan S. Marvin, Loren Looger
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Abstract
The present disclosure provides, inter alia, genetically encoded recombinant peptide biosensors comprising analyte-binding framework portions and signaling portions, wherein the signaling portions are present within the framework portions at sites or amino acid positions that undergo a conformational change upon interaction of the framework portion with an analyte.
Claims32
A recombinant peptide biosensor comprising an analyte-binding framework portion and a signaling portion, wherein the signaling portion is present within the framework portion at a site or amino acid position that undergoes a conformational change upon interaction of the framework portion with a defined, specific, or selected analyte, wherein the recombinant peptide biosensor comprises a amino acid sequence having at least 85% sequence identity to SEQ ID NO:
- 184.
A recombinant peptide biosensor comprising an amino acid sequence having at least 90% sequence identity to SEQ ID NO:184, wherein the recombinant peptide biosensor binds specifically to GABA.
A method for detecting GABA, the method comprising detecting a level of fluorescence emitted by a recombinant peptide biosensor, the peptide biosensor having the amino acid sequence shown in SEQ ID NO:184, and correlating the level of fluorescence with the presence of GABA.
Dependencies are inferred from the claim text.