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US 11,698,364 B2Granted
Real-time cell-surface marker detection
AssigneeUniversity of Washington
InventorsThomas J. Matula, Masaoki Kawasumi, Oleg Sapozhnikov
Life of this patent
Estimating the term…
Abstract
Cell-separation systems and methods utilizing cell-specific microbubble tags and ultrasound-based separation are described. The methods are useful for simplification of time-consuming and costly cell purification procedures and real time apoptosis detection.
Claims10
A cell-sorting system comprising:
- a flow cell for flowing a sample comprising microbubble-labeled cells and unlabeled cells, wherein the flow cell comprises one or more inlet channels, a flow channel having an upstream portion, upstream of one or more acoustic transducers relative to a direction of sample flow through the flow channel, and a downstream portion, downstream of the one or more acoustic transducers relative to a direction of sample flow through the flow channel, and one or more outlet channels;
- and
- the one or more acoustic transducers acoustically coupled with the flow cell, wherein the one or more acoustic transducers is positioned and configured to deliver a traveling acoustic wave through the flow channel to apply an acoustic radiation force to a sample flowing through the flow channel such that the microbubble-labeled cells are displaced relative to unlabeled cells along a direction of the acoustic radiation force away from the one or more acoustic transducers, the direction of the acoustic radiation force being transverse to the direction of sample flow.
Dependencies are inferred from the claim text.