Method of purity determination by capillary electrophoresis

A method for improving protein purity determination by capillary electrophoresis, comprising separating a denatured protein of interest in a sample in a buffer composition comprising (i) a hydrophobic detergent comprising a sulfate head group and a hydrophobic tail comprising an alkyl chain having greater than 14 carbon atoms, and (ii) an organic additive which comprises mannitol, glycerol, ethylene glycol, ethanol, methanol, or any combination thereof.

An electrophoresis buffer composition comprising (i) a hydrophobic detergent comprising a sulfate head group and a hydrophobic tail comprising an alkyl chain having greater than 14 carbon atoms, (ii) an organic additive which comprises mannitol, glycerol, ethylene glycol, ethanol, methanol, or any combination thereof, and (iii) at least one component selected from (a) a hydrophilic polymer which comprises dextran, polyacrylamide, polyethylene glycol, or any combination thereof and (b) a metal chelator which comprises ethyl enedi ami netetraacetic acid, diethylenetriaminepentaacetic acid, or any combination thereof.

A method for separating a protein of interest in a sample via electrophoresis, comprising denaturing the protein of interest in a buffer composition comprising i) a hydrophobic detergent comprising a sulfate head group and a hydrophobic tail comprising an alkyl chain having greater than 14 carbon atoms, and (ii) an organic additive which comprises mannitol, glycerol, ethylene glycol, ethanol, methanol, or any combination thereof.

A method for improving peak separation efficiency of a protein of interest in a sample via electrophoresis, comprising denaturing the protein of interest in a buffer composition comprising (i) a hydrophobic detergent comprising a sulfate head group and a hydrophobic tail comprising an alkyl chain having greater than 14 carbon atoms, and (ii) an organic additive which comprises mannitol, glycerol, ethylene glycol, ethanol, methanol, or any combination thereof.