Method for screening, isolating and purifying analytes

A method for screening, isolating and purifying an analyte with an optically induced-electrophoresis (ODEP) device, comprising the steps of:

• (a) performing a treatment on the analyte to obtain a treated analyte, wherein the treatment comprises a background solution directed towards the analyte, and produces a difference in size or degree of cell viability in the analyte;
• (b) introducing the treated analyte into the optically induced-electrophoresis device, wherein the optically induced-electrophoresis device comprises a main microchannel, at least one side microchannel, at least one target collection tank, and an optically-induced dielectrophoretic force light image module having a controllable velocity, wherein the optically-induced dielectrophoretic force light image module screens the treated analyte in the main microchannel in order to separate the treated analyte according to its size or degree of cell viability into at least one target using a separation and purification method;
• and
• (c) collecting the at least one target into the at least one target collection tank;
• wherein the optically-induced dielectrophoretic force light image module comprises at least one moving light bar selected from the group consisting of:
• first moving light bar, second moving light bar, and a combination thereof;
• wherein the at least one first moving light bar has a moving velocity of a light image ranging from 0.01 pm/s to 1 cm/s, the moving velocity of the light image is a variable speed, and the moving velocity of the light image increases when the at least one first moving light bar moves a predetermined distance;
• wherein the optically-induced dielectrophoretic force light image module comprises at least one second moving light bar, the at least one second moving light bar has a moving velocity of a light image ranging from 0.01 pm/s to 1 cm/s, the moving velocity of the light image among the at least one second moving light bar is different, and the moving velocity of the light image is a constant speed;
• wherein the size or degree of cell viability of the analyte is screened, and the analyte having different sizes or degrees of viability is isolated and purified by the ODEP device;
• and
• wherein cells having different degrees of environmental tolerance gene expression are screened, isolated and purified depending on their environmental stimulus-induced differences of cell viability degrees by the ODEP device.