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US 11,697,853 B2Granted
Detecting prostate cancer
AssigneesMayo Foundation for Medical Education and Research, Exact Sciences Corporation
InventorsDavid A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney, Brian A. Dukek, Matthew T. Gettman, Hatim T. Allawi
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Abstract
Provided herein is technology for prostate cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of prostate cancer.
Claims6
A method comprising:
- extracting genomic DNA from a biological sample of a human individual;
- treating the extracted genomic DNA with bisulfite;
- amplifying the bisulfite-treated genomic DNA using primers specific for at least one CpG site in GAS6 and primers specific for at least one CpG site in ITPRIPL1, wherein the primers specific for the at least one CpG site in GAS6 are capable of binding an amplicon bound by a sequence comprising SEQ ID NOs:
- 117 and 118 or SEQ ID NOs:
- 165 and 166, and wherein the primers specific for the at least one CpG site in ITPRIPL1 are capable of binding an amplicon bound by a sequence comprising SEQ ID NOs:
- 45 and 46 or SEQ ID NOs:
- 195 and 196;
- and
- measuring a methylation level of the at least one CpG site in GAS6 and the at least one CpG site in ITPRIPL1 using methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, or bisulfite genomic sequencing PCR.
6 more elements
Dependencies are inferred from the claim text.