Reagent and method for fluorescence quantitative real-time PCR detection of RCL

A reagent combination comprising:

• (i) a first primer pair for specific amplification of vesicular stomatitis virus G protein (VSV-G) gene and a first probe,
• wherein, the first primer pair comprises:
• a first forward primer consisting of the sequence of SEQ ID NO:
• 1, and a first reverse primer consisting of the sequence of SEQ ID NO:2;
• wherein the first probe consists of the sequence of SEQ ID NO:
• 3 and a first detectable label;
• and
• (ii) a second primer pair for specific amplification of VSV-G gene and a second probe,
• wherein, the second primer pair comprises:
• a second forward primer consisting of the sequence of SEQ ID NO:
• 4, and a second reverse primer consisting of the sequence of SEQ ID NO:
• 5;
• wherein the second probe consists of the sequence of SEQ ID No:
• 6 and a second detectable label.