Oligonucleotide-functionalized hydrophobic polymer nanoparticles

A dye-loaded polymeric nanoparticle comprising:

• a hydrophobic polymer chain, said chains bearing at least one moiety of formula (I),
• wherein:
• A represents a spacer chosen from:
• —(CH2)m—, —(CH2)pNH—CO(CH2)q—, —(CH2)pCO—NH(CH2)q—, —(CH2)pO(CH2)q—, —(CH2)pNH(CH2)qO—, —(CH2)mCO—, —CO(CH2)m—, each of m, p and q represent independently from each other an integer chosen from 0 to 8, preferably an integer chosen from 0 to 5, more preferably an integer chosen from 0 to 3,
• D represents a group chosen from:
• wherein R′ represents a hydrogen, a halogen, a (C1-C8)alkyl,
• a cyclo(C3-C7)alkyl eventually monosubstituted, a monocyclic non-aromatic heterocyclic group eventually monosubstituted, or a monocyclic aromatic group eventually monosubstituted,
• E represents Y—NRa, where Ra is H or an (C1-C8)alkyl,
• X, Y, Z are identical or different and each represent independently of the other a spacer chosen from —(CH2)r—, —(CH2—CH2—O)r, —(CH2—CH2—NH)r—, —(CH2)sNH—CO(CH2)t—, —(CH2)sCO—NH(CH2)t—, wherein r, s and t represent independently from each other an integer chosen from 0 to 8, preferably an integer chosen from 0 to 3,
• n represents an integer chosen from 1 to 10, in particular 1 to 3 and wherein at least one of m, p, q, r, s and t have a value different from 0,
• when D represents
• E can be absent;
• said moiety of formula (I) being bound to a carboxyl group of the polymer and being situated on the surface of the nanoparticle and
• energy donors formed by a salt of at least one donor dye and bulky fluorinated anion, said energy donors being encapsulated in the hydrophobic polymer.