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US 11,697,831 B2Granted
Cell able to produce poly l-lactic acid
AssigneesUniversita Degli Studi Di Milano-Bicocca, Galatea Biotech S.R.L.
InventorsDanilo Porro, Paola Branduardi, Stefano Bertacchi, Nadia Maria Berterame
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Abstract
Disclosed is the production by fermentation of poly D-lactic acid (PDLA) and poly L-lactic acid (PLLA). In particular, there is provided engineered (prokaryotic or eukaryotic) cells for the direct synthesis of PLLA polymers and engineered eukaryotic cells for the direct synthesis of PDLA polymers starting from a carbon source, including residual biomasses of the different production chains.
Claims12
A cell able to produce poly-L-lactic acid (PLLA), wherein said cell comprises:
- (i) a L-lactate dehydrogenase enzyme encoded by the Lactobacillus plantarum L-lactate dehydrogenase ldh 1 gene having the nucleotide sequence of SEQ ID NO:4, wherein said L-lactate dehydrogenase enzyme catalyzes the conversion of pyruvate into L-lactate;
- (ii) an acyl-CoA transferase enzyme which is a propionyl-CoA transferase enzyme encoded by a gene having the nucleotide sequence of SEQ ID NO:2, wherein said gene is a mutated form of the Clostridium propionicum propionyl-CoA transferase gene, and wherein said acyl-CoA transferase enzyme catalyzes the synthesis of L-lactoyl-CoA by thioesterification of L-lactate;
- and
- (iii) a polyhydroxyalkanoate synthase enzyme encoded by a gene having the nucleotide sequence of SEQ ID NO:3, wherein said gene is a mutated form of the Pseudomonas resinovorans polyhydroxyalkanoate synthase C1 gene, and wherein said polyhydroxyalkanoate synthase enzyme catalyzes the polymerization of L-lactoyl-CoA into PLLA;
- wherein said cell able to produce PLLA is a yeast cell.
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Dependencies are inferred from the claim text.