Chemoautotrophic cells comprising an engineered carbon fixation pathway
AssigneeGinkgo Bioworks, Inc.
InventorsCurt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
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Abstract
The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
Claims18
A chemoautotrophic host cell that comprises a heterologous polynucleotide encoding a hexulose-6-phosphate synthase (HPS), wherein the polynucleotide comprises a sequence that is at least 90% identical to SEQ ID NO:
- 46 or 47.
A polynucleotide comprising a sequence that is at least 90% identical to SEQ ID NO:
- 46 or 47.
A chemoautotrophic host cell that comprises a heterologous polynucleotide encoding a hexulose-6-phosphate synthase (HPS), wherein the HPS comprises a sequence that is at least 90% identical to the Methylococcus capsulatus HPS protein encoded by SEQ ID NO:
- 46 or 47, wherein the HPS protein encoded by SEQ ID NO:
- 46 corresponds to NCBI Reference Sequence YP_115138 and wherein the HPS protein encoded by SEQ ID NO:
- 47 corresponds to NCBI Reference Sequence YP_115430.
Dependencies are inferred from the claim text.