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US 11,697,824 B2Granted
Vector for the production of AAV particles
AssigneeOxford Genetics Limited
InventorsRyan Cawood, Alissa Sarah Bray, Thomas Augustus Payne
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Abstract
The present invention relates to the production of plasmids which are useful in the production of Adeno-Associated Virus (AAV) particles. In particular, the invention provides nucleic acid molecules comprising capgenes and repgenes, wherein the capand repgenes are both operably-associated with the same promoter. The invention also provides host cells comprising nucleic acid molecules of the invention and methods for their use.
Claims18
A nucleic acid molecule comprising:
- (i) a promoter;
- (ii) an AAV cap gene;
- and
- (iii) an AAV rep gene, encoding one or more Rep proteins selected from the group consisting of Rep78, Rep68, Rep52 and Rep40,
- in the above 5′→3′ order, wherein the cap gene and the rep gene are both operably-associated with the promoter, and wherein the rep gene is also operably-associated with an IRES (Internal Ribosome Entry Site).
2 more elements
An RNA molecule comprising:
- (i) an AAV cap gene;
- and
- (ii) an AAV rep gene,
- in the above 5′→3′ order, wherein the rep gene is operably-associated with an IRES.
1 more element
Dependencies are inferred from the claim text.