Blue aleurone and other segregation systems

A cereal plant for use in the production of hybrid cereal plants, wherein the cereal plant comprises at least one homoeologous chromosome pair, wherein the pair consists of a first and second chromosome,

• wherein the first chromosome is native to the cereal plant and the second chromosome comprises an alien chromosome fragment comprising a dominant male fertility restorer gene and at least one selection marker gene on the same side of a centromere of the second chromosome,
• wherein the cereal plant comprises a male fertility gene mutation causing male sterility,
• wherein the at least one selection marker gene is non-transgenic,
• wherein the male fertility restorer gene is from Triticum boeoticum or Triticum monococcum and comprises a nucleic acid sequence selected from the group consisting of:
• (i) a nucleic acid sequence as set forth in SEQ ID NO:
• 1, 6, 7, 8, or 10, or fragments or variants thereof that produce functional amino acid sequences;
• (ii) a nucleic acid sequence with at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the nucleic acid sequence as set forth in SEQ ID NO:
• 1, 6, 7, 8, or 10, or fragments thereof that produce functional amino acid sequences;
• (iii) a nucleic acid sequence having a coding sequence as set forth in SEQ ID NO:
• 2, 4, 9, 11, or 14, or fragments or variants thereof that produce functional amino acid sequences;
• (iv) a nucleic acid sequence having a coding sequence with at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the nucleic acid sequence as set forth in SEQ ID NO:
• 2, 4, 9, 11, or 14, or fragments thereof that produce functional amino acid sequences;
• (v) a nucleic acid sequence encoding an amino acid sequence as set forth in SEQ ID NO:
• 3, 5, 15, 42, or 43, or fragments or variants thereof that produce functional amino acid sequences;
• and
• (vi) a nucleic acid sequence encoding an amino acid sequence with at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the amino acid sequence as set forth in SEQ ID NO:
• 3, 5, 15, 42, or 43, or fragments thereof, and
• wherein the at least one selection marker gene is a blue aleurone gene, wherein the blue aleurone gene is from Agropyron elongatum, Agropyron trichophorum, or Triticum monococcum, and wherein the blue aleurone gene comprises a nucleic acid sequence selected from the group consisting of:
• (i) a nucleic acid sequence having a coding sequence of SEQ ID NO:
• 44 or 12, or fragments or variants thereof that produce functional amino acid sequences;
• (ii) a nucleic acid sequence having a coding sequence with at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the nucleic acid sequence of SEQ ID NO:
• 44 or 12, or fragments thereof that produce functional amino acid sequences;
• (iii) a nucleic acid sequence encoding an amino acid sequence of SEQ ID NO:
• 45 or 13, or fragments or variants thereof that produce functional amino acid sequences;
• and
• (iv) a nucleic acid sequence encoding an amino acid sequence with at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the amino acid sequence of SEQ ID NO:
• 45 or 13, or fragments thereof.