Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsOded Shoseyov, Helena Magrisso, Tzvi Zvirin, Amit Yaari, Zohar Katz
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Abstract
A method of modifying a glycosylation pattern of a polypeptide-of-interest in a plant or plant cell is provided. The method comprising expressing in a plant or plant cell transformed to express at least one glycosidase in a subcellular compartment, a nucleic acid sequence encoding the polypeptide-of-interest, such that the at least one glycosidase and the polypeptide-of-interest are co-localized to the subcellular compartment of the plant or plant cell, thereby modifying the glycosylation pattern of the polypeptide-of-interest in the plant or plant cell.
Claims19
A method for producing a human polypeptide-of-interest with a modified glycosylation pattern, the method comprising introducing into a plant or plant cell:
- a nucleic acid sequence encoding the human polypeptide-of-interest translationally fused to a signal peptide for localization in a subcellular compartment;
- one or more agents for suppressing expression of a fucosyltransferase and a xylosyltransferase;
- at least one polypeptide which confers an agricultural effective trait;
- and
- a nucleic acid sequence encoding an affinity moiety translationally fused to a cell wall binding peptide or a signal peptide for localization in the subcellular compartment,
- wherein the affinity moiety is for binding the human polypeptide of interest, and wherein the subcellular compartment is the apoplast or the endoplasmic reticulum.
A plant or plant cell characterized by:
- (i) the plant expressing a nucleic acid sequence encoding a human polypeptide of interest translationally fused to a signal peptide for localization in a subcellular compartment;
- (ii) the plant having reduced endogenous levels of a fucosyltransferase and a xylosyltransferase;
- (iii) the plant expressing a nucleic acid sequence encoding an affinity moiety translationally fused to a cell wall binding peptide or to a signal peptide for localization in the subcellular compartment, wherein the affinity moiety is for binding the human polypeptide of interest;
- and
- (iv) the plant expressing at least one nucleic acid sequence encoding a polypeptide which confers an agricultural effective trait;
- wherein the subcellular compartment is the apoplast or the endoplasmic reticulum.
Dependencies are inferred from the claim text.