Genus Hydrogenophilus bacterium transformant
AssigneesUtilization of Carbon Dioxide Institute Co., Ltd., The University of Tokyo
InventorsHideaki Yukawa, Naoto Ohtani, Masaharu Ishii
Life of this patent
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Abstract
A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source. (a1) DNA which consists of a base sequence of SEQ ID NO: 1; (a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity; (a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.
Claims19
A synthesized DNA encoding 2-keto-acid decarboxylase of (a1) or (a2):
- (a1) comprising a base sequence of SEQ ID NO:
- 1;
- or
- (a2) comprising a base sequence having 90% or more identity with SEQ ID NO:
- 1 and being configured to be expressed in bacteria of genus Hydrogenophilus.
A synthesized DNA encoding 2-keto-acid decarboxylase comprising a base sequence of SEQ ID NO:
- 1.
A synthesized DNA for recombinant expression of 2-keto-acid decarboxylase in a bacterium of genus Hydrogenophilus, comprising a base sequence having 90% or more identity with SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.