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US 11,697,809 B2Granted
Biological synthesis of amino acid chains for preparation of peptides and proteins
AssigneeChristoph Kutzner
InventorsChristoph Kutzner, Marco Giuman
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Abstract
The invention relates to fusion polypeptides, nucleic acid molecules encoding said fusion polypeptides and genetically modified cells including said nucleic acid molecules. Moreover, the invention relates to a method for preparing target polypeptides using the fusion polypeptides.
Claims10
A fusion polypeptide comprising in direction from the N-terminus to the C-terminus:
- (i) a purification domain,
- (ii) an autoprotease domain, and
- (iii) a target peptide domain,
- wherein the purification domain (i) binds to cellulose, chitin, and/or starch and comprises a glucoamylase and/or an amylase, and
- wherein the autoprotease domain (ii) comprises Classical Swine Fever Virus (CSFV) Npro mutant EDDIE, wherein the CSFV Npro mutant EDDIE comprises the amino acid sequence of SEQ ID NO:
- 14, and wherein the autoprotease domain (ii) cleaves the fusion polypeptide after the C-terminus of the autoprotease domain (ii) and before the N-terminus of the target peptide domain (iii).
3 more elements
Dependencies are inferred from the claim text.