Enhanced differentiation and maturation of pluripotent stem cell-derived myogenic cells
AssigneeRegents of the University of Minnesota
InventorsRita Perlingeiro, Sridhar Selvaraj, Ricardo Mondragon Gonzalez
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Abstract
A method to increase the efficiency of myotube generation and maturation from pluripotent stem cells comprising: (a) differentiating pluripotent stem cells to myogenic progenitors; and (b) terminally differentiating said myogenic progenitors from (a) into myotubes in the presence of at least one gamma secretase inhibitor, wherein myotube generation is increased in the presence of at least one gamma secretase inhibitor, as compared to differentiation in the absence of gamma secretase inhibitors.
Claims8
An in vitro method of obtaining myotubes comprising:
- (a) differentiating pluripotent stem cells into myogenic progenitors;
- (b) terminally differentiating said myogenic progenitors from (a) into mature myotubes in the presence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F);
- and
- (c) isolating said myotubes.
An in vitro method to increase the efficiency of myotube generation and maturation from pluripotent stem cells comprising:
- (a) differentiating pluripotent stem cells to myogenic progenitors;
- and
- (b) terminally differentiating said myogenic progenitors from (a) into mature myotubes in the presence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F),
- wherein myotube maturation is increased in the presence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F), as compared to maturation in the absence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F).
An in vitro method to generate myotubes comprising:
- (a) differentiating pluripotent stem cells into myogenic progenitors;
- and
- (b) terminally differentiating said myogenic progenitors from (a) into mature myotubes in the presence of a combination of DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F).
A composition comprising myogenic progenitors, DAPT (Da), SB-431542 (S), dexamethasone (De) and forskolin (F).
Dependencies are inferred from the claim text.